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| Costa,Maria Regina de Macedo; Ribeiro,Camila Godinho; Santos-Filho,Sebastião David; Neves,Rosane de Figueiredo; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario; Catanho,Maria Teresa Jansem de Almeida. |
| The development of experimental assays to study properties of herbal medicine is worthwhile. Vitex agnus castus (VAC) is utilized in popular medicine and some actions have been attributed to its extract. Blood cells (BC) and plasma proteins are labeled with technetium-99m (Tc-99m) and have been used in nuclear medicine, as in basic research. This procedure uses a reducing agent and stannous ion is utilized. There are reports that drugs can alter this labeling process. The aim of this work was to evaluate the influence of an aqueous extract of VAC on the labeling of blood constituents with Tc-99m. Blood was incubated with VAC, stannous chloride and Tc-99m, as sodium pertechnetate, and centrifuged. Samples of BC and plasma were separated, aliquots of BC and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oxidant properties; Plasma proteins; Red blood cells; Technetium-99m; Vitex agnus-castus. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600023 |
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| Mousinho,Kristiana Cerqueira; Correia,Marília Bezerra Libório; Silva,Jailson Oliveira da; Magnata,Simey de Souza Leão Pereira; Souza,Ivone Antônia de; Catanho,Maria Teresa Jansem de Almeida. |
| The aim of this study was to evaluate the influence of the proteic extract of R. communis on the cell physiology by the osmotic fragility, labeling of the blood elements with the 99mTc and cell morphology. To evaluate the osmotic fragility, the blood samples of the Wistar rats were incubated with the concentrations of R. communis and with the solutions of NaCl (0.4; 0.7; 0.9%). In the labeling of the blood elements procedure, the rat blood was treated with a solution of Tc-99m and TCA at 5%, determining the rate of radioactivity (%ATI) in the plasma (P) and in the red blood cells (RBC). The soluble and insoluble fractions of the plasma were also evaluated. The cells morphology submitted to the extract was evaluated by the optical microscopy (x40). The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ricinus communis L.; Labeling of red blood cells; Osmotic fragility; Technetium-99m; Morphology of cells. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600008 |
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